anti-ROMs Test

The anti-ROMs Test – newer in terms of development than BAP Test and OXY-Adsorbent Test – it is somewhat an evolution of these.

It is a photometric test used to determine the quantity of fast anti-oxidants (for example Vitamin C, Vitamin E) and slow anti-oxidants (for example uric acid, thiols) dividing the two categories.

The principle

The anti-ROMs Test is proposed as an evolution of BAP Test that preserves, however, its validity and its specific indications. The principle is essentially the same because both the tests measure the antioxidant capacity of plasma in terms of iron-reducing capacity.
The innovative feature that distinguishes the anti-ROMs Test is that it relies on the development of a response in two sequential stages. The first stage measures the iron-reducing capacity of antioxidants "rapid", such as vitamins C and E, which intervene immediately to eliminate any oxidant species. The second stage measure that of anti-oxidants "slow", such as uric acid and certain thiols ( e.g. cysteine), which come into play late in the defense against free radicals.

Working Conditions

The test must be carried on heparinized plasma or serum.

It can be performed both with the dedicated systems (FREE CarpeDiem or FREE Duo) and with standard manual photometers.

Interpretation of results

The anti-ROMs Test gives two distinct results: in normal conditions, a value of 200 µEq/L for fast anti-oxidants and 1000 µEq/L for slow anti-oxidants is considered optimal. Lower values suggest an oxidative stress condition.
With this separated evaluation of antioxidant defenses the clinician can offer his patient a more precise description of his antioxidant potential.
A low value of the rapid antioxidants (<200 µEq/L) suggest a condition of reduced bioavailability of ascorbate and/or tocopherols and/or polyphenols. It will stimulate the research of causes of alteration and then be reflected in practice in the implementation of strategies aimed to the immediate correction of the defect through a higher intake of micronutrients (food and/or nutritional supplementation).
Instead, the value of antioxidants slow, if less than 1000 µEq/L, will be considered as indicative of an insufficient supply of thiols (eg. cysteine, lipoic acid). This may indicate poor nutrition in animal protein (for uric acid) or of a lack of physical activity. This value may then be confirmed by the –SHp Test.
It is interesting to note that the value of the rapid antioxidant is sensitive to the assumption of any supplements and for this reason it can be used just to monitor the effectiveness of foods and/or formulations that are intended to increase the efficiency of defense systems designed to counter the oxidative insults. A low result of low antioxidants, however, would indicate a reduced antioxidant capacity of endogenous systems that are more sensitive to endogenous metabolism and physical activity.

ABOUT US

DIACRON INTERNATIONAL s.r.l is the leader company in research and evaluation of oxidative stress. Our company produces and sells analytical dedicated instruments, kits and accessories for oxidative stress assessment in Human, Veterinary and Agrifood fields.

The company was founded in 2001 by  Mauro Carratelli, the inventor of d-ROMs Test and of all the dedicated kits for oxidative stress assessment (Panel Carratelli). He is the current President and he leads the Research team. The Carratelli’s vision is to allow the assessment of the oxidative stress in the clinical practice, available to every people involved in healthcare.

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