OXY-Adsorbent Test

The OXY-Adsorbent Test is a photometric test that measures the blood concentration of antioxidant compounds scavengers (as in the BAP Test), but also identifies shocks adsorbers. The latter are all antioxidants not active from the chemical point of view, but able to “plug” the oxidant action of ROS, so representing “sacrificial” elements.

Amino acids, proteins, mucopolysaccharides and potentially all organic molecules belongs to this group of antioxidants while bilirubin, uric acid, vitamins C and E, albumin and, in General, macromolecular complexes that act as a shock absorber against free radicals, such as glycoproteins, helps curbing the oxidizing action of hypochlorous acid.

Principle

Many substances in the plasma are able to curb the potential oxidizing action of reactive oxygen species. Virtually every agent, whether it be endogenous (eg. GSH, protein, bilirubin, uric acid, cholesterol, etc.) or exogenous (e.g. carotenoids, ascorbate, vitamin E, etc.) can donate electrons blocking the potential damaging of free radicals, whose reactivity is linked to particular deficiency of these tiny particles.
The OXY-Adsorbent Test assesses the ability of the plasma to oppose the massive oxidizing by a solution of hypochlorous acid (HClO) because this is an antioxidant not only powerful but also physiological and therefore better able to mimic situations that occur in vivo.
Bilirubin, uric acid, vitamins C and E, albumin and, in General, macromolecular complexes that act as a shock absorber against free radicals, such as glycoproteins, helps curbing the oxidizing action of hypochlorous acid.

Reference values

According to the literature, the reference values of the test for healthy subjects is higher than 350 μmol HClO/L of sample.

Values below 350 micromole of HClO suggest a reduction of the anti-oxidant barrier, (therefore an oxidative stress condition) and correlates directly with the damage absorbed.

Indeed, the higher will be the excess of free radicals detected, the more the plasma barrier will be compromised.

μmol HClO/ml
of sample
Impairment degree
of antioxidant barrier
> 350 Normal range
350 - 320 Slight reduction
319 - 280  Moderate reduction
279 - 250 Strong reduction
< 250 Very strong reduction

Working conditions  

OXY-Adsorbent Test can be carried out either heparinized plasma or serum. Serum and heparinized plasma can be used fresh or frozen at -20°C. It can be carried both on dedicated systems (FREE Carpe Diem and FREE DUO) and on standard photometer or automatic analyzers.
On the basis of the published data the OXY-Adsorbent Test is a reliable, accurate and repeatable Test.


Clinical studies

The OXY-Adsorbent Test has proven very reliable in determining the total plasma antioxidant capacity in several clinical studies, both in human and veterinary medicine. This test integrate the results of d-ROMs Test in the global assessment of oxidative stress, especially in cases of high levels of reactive oxygen metabolites (ROM) in the serum.

However not necessarily an high value of d-ROMs Test correspond a lower value of OXY-Adsorbent Test because next to the classical situation in which the increase of reactive species is secondary to a reduced efficiency of the plasma barrier, there is the possibility of an increased production of free radicals as a primary event.

Relevant uses

The OXY-Adsorbent Test is used for numerous applications in the field of human medicine but also in veterinary medicine, in many animal species, from chickens to rabbits, from pigs to cattle.

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DIACRON INTERNATIONAL s.r.l is the leader company in research and evaluation of oxidative stress. Our company produces and sells analytical dedicated instruments, kits and accessories for oxidative stress assessment in Human, Veterinary and Agrifood fields.